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her2 neu  (Cell Marque)

 
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    Cell Marque her2 neu
    Her2 Neu, supplied by Cell Marque, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/her2+neu/pm42086287-47-61-64?v=Cell+Marque
    Average 86 stars, based on 1 article reviews
    her2 neu - by Bioz Stars, 2026-06
    86/100 stars

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    Construction of IF + FISH detection method by TSA IF + FISH detection system was constructed by using anti-linear antigen <t>HER2</t> antibody and the TSA method before or after denaturation on SKBR3. Centromeric probes of chromosome 10q22.3 (yellow), 10cen (blue), and chromosome 3p22.1 (red) and 3q29 (green) were used for FISH detection. Results demonstrated that cells maintained a remarkable detection signal even after denaturation when using TSA-488 (test with HRP labeled anti HER2 antibody), TSA-568 (test with HRP labeled anti-EpCAM antibody), and TSA-594 (test with HRP labeled anti pan CK antibody) (A) Overcoming Antigen Degradation in IF-FISH Assays through Tyramide Signal Amplification (TSA). (B) Application of TSA-IF-FISH for Enhanced Detection of HER2, EpCAM, and CK and Chromosomal Abnormalities in Circulating Tumor Cells. Bars: 8 μm. HER2, Human epidermal growth factor receptor 2; EpCAM, Epithelial cell adhesion molecule.
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    Construction of IF + FISH detection method by TSA IF + FISH detection system was constructed by using anti-linear antigen HER2 antibody and the TSA method before or after denaturation on SKBR3. Centromeric probes of chromosome 10q22.3 (yellow), 10cen (blue), and chromosome 3p22.1 (red) and 3q29 (green) were used for FISH detection. Results demonstrated that cells maintained a remarkable detection signal even after denaturation when using TSA-488 (test with HRP labeled anti HER2 antibody), TSA-568 (test with HRP labeled anti-EpCAM antibody), and TSA-594 (test with HRP labeled anti pan CK antibody) (A) Overcoming Antigen Degradation in IF-FISH Assays through Tyramide Signal Amplification (TSA). (B) Application of TSA-IF-FISH for Enhanced Detection of HER2, EpCAM, and CK and Chromosomal Abnormalities in Circulating Tumor Cells. Bars: 8 μm. HER2, Human epidermal growth factor receptor 2; EpCAM, Epithelial cell adhesion molecule.

    Journal: iScience

    Article Title: Analyzed PD-L1-positive subpopulations by dual-labeling TSA-IF-FISH predicts immunotherapy efficacy in advanced lung cancer

    doi: 10.1016/j.isci.2025.114357

    Figure Lengend Snippet: Construction of IF + FISH detection method by TSA IF + FISH detection system was constructed by using anti-linear antigen HER2 antibody and the TSA method before or after denaturation on SKBR3. Centromeric probes of chromosome 10q22.3 (yellow), 10cen (blue), and chromosome 3p22.1 (red) and 3q29 (green) were used for FISH detection. Results demonstrated that cells maintained a remarkable detection signal even after denaturation when using TSA-488 (test with HRP labeled anti HER2 antibody), TSA-568 (test with HRP labeled anti-EpCAM antibody), and TSA-594 (test with HRP labeled anti pan CK antibody) (A) Overcoming Antigen Degradation in IF-FISH Assays through Tyramide Signal Amplification (TSA). (B) Application of TSA-IF-FISH for Enhanced Detection of HER2, EpCAM, and CK and Chromosomal Abnormalities in Circulating Tumor Cells. Bars: 8 μm. HER2, Human epidermal growth factor receptor 2; EpCAM, Epithelial cell adhesion molecule.

    Article Snippet: Alexa Fluor 488–conjugated anti-HER2 antibody , Santa Cruz Biotechnology , sc-7301 AF488.

    Techniques: Construct, Labeling, Amplification